Table 1 |
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|
BAC libraries screening. cDNA probes used were either involved in the lignin biosynthetic pathway (PAL, C4 H, HCT, C3 H, CCoAOMT, CCR, F5H/CAld5H, CAD) or regulating this pathway (MYB1 and 2, RAC1). |
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|
Gene family |
Gene |
EMBL accession |
library |
Positive clones Array hybridization (a) |
PCR (b) |
Ratio (b/a) % |
|
|
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|
Phenylalanine ammonia lyase (PAL) |
EguPAL_ |
EG__Ba |
15 |
11 |
63 |
|
|
Cinnamate 4-hydroxylase (C4H) |
EguC4H |
EG__Bb |
6 |
2 |
33 |
|
|
4 coumarate CoA ligase (4CL) |
Egu4CL |
EG__Bb |
15 |
12 |
80 |
|
|
Hydroxycinnamoyl-CoA:shikimate/quinate hydroxycinnamoyl transferase (HTC) |
EguHTC |
EG_Ba |
13 |
13 |
100 |
|
|
p-coumarate 3-hydroxylase (C3H) |
EguC3H |
EG__Bb |
5 |
3 |
60 |
|
|
Caffeoyl-CoA O-methyltransferase (CCoaOMT) |
EguCCoaOMT |
EG_Ba |
19 |
7 |
37 |
|
|
Cinnamoyl CoA reductase (CCR) |
EguCCR |
EG_Ba |
13 |
10 |
77 |
|
|
Ferulate 5 hydroxylase/coniferaldehyde 5 hydroxylase (F5H/CAld5H) |
EguF5H |
Eg_Ba |
17 |
5 |
29 |
|
|
Caffeic acid/5- hydroxyoniferaldehyde O-methyltransferase (COMT) |
EguCOMT |
EG__Bb |
19 |
12 |
63 |
|
|
Cinnamyl Alcohol dehydrogenase |
EguCAD2 |
EG__Ba |
22 |
13 |
59 |
|
|
Rho-related small GTP-binding protein |
EguRAC1 |
EG__Bb |
33 |
3 |
9 |
|
|
R2R3 MYB transcription factor |
EguMyb1 |
EB_Ba |
10 |
9 |
90 |
|
|
R2R3 MYB transcription factor |
EguMyb2 |
EG__Bb |
7 |
6 |
86 |
|
|
|
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|
Screening was made on subsets of EG_Ba and EG_Bb BAC libraries with redundant genome coverage of 8.5× and 7.5×, respectively. Number of positive clones obtained (a) and validated by PCR (b). |
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|
Paiva et al. BMC Genomics 2011 12:137 doi:10.1186/1471-2164-12-137 |
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