Confirmation of microarray results using quantitative real-time PCR. (A), Raw intensity values were measured using microarray. (B), Real-time PCR results for the same genes are expressed as the relative mRNA expression level normalized by GAPDH mRNA level. The abundance of mRNA in the controls was set at 1. Data represent mean ± SD of 4 replicates. Ctr, Control; Cyto, Cytokines; shRNA, HNF4α shRNA; shRNA+Cyto, HNF4α shRNA plus Cytokines. *p < 0.05 and **p < 0.01 indicate a significant difference compared to control. Gene abbreviations are the same as described in Figure 3 legend.
Wang et al. BMC Genomics 2011 12:128 doi:10.1186/1471-2164-12-128