Knock-down of HNF4α in HepG2 cells. HepG2 cells were transfected with non-specific shRNA control or HNF4α shRNA plasmid. mRNA and whole cell lysates were prepared for real-time PCR (A) and Western blots (B), respectively. The results shown in (A) represent the relative mRNA expression level normalized to GAPDH mRNA level. The abundance of mRNA in the controls was set at 1. Data represent mean ± SD of 4 replicates. An HNF4α antibody (sc-6556, Santa Cruz Biotechnology) and β-actin (Sigma) antibody, used as an internal loading control, were utilized for Western blot (B).
Wang et al. BMC Genomics 2011 12:128 doi:10.1186/1471-2164-12-128