Table 4

Real time RT-PCR expression analysis of selected Fusarium oxysporum f. sp. melonis (FOM) genes.

ID

Homology

E-value

Annotation

ΔCt

SD

P493

FOXT_12671

5E-152

Fusarium oxysporum f.sp lycopersici aspartate aminotransferase

3.43

0.11

P135

FOXT_08523

1E-48

Fusarium oxysporum f.sp lycopersici acyl-CoA desaturase

16.11

0.41

P1315

FOXG_05190

2E-79

Fusarium oxysporum f.sp lycopersici E3 ubiquitin-protein ligase

3.99

0.31

P819

FOXG_09125

2E-117

Fusarium oxysporum f.sp lycopersici hypothetical protein similar to avenacinase

10.24

0.04

P1403

FOXT_13204

7E-51

Fusarium oxysporum f.sp lycopersici predicted protein

7.06

0.77

Five Fusarium oxysporum f. sp. melonis transcripts, expressed in planta during infection according to cDNA-AFLP experiments, were selected for validation by Real time RT-PCR. Data are shown as the difference between the Ct of the gene analyzed and the Ct of melon actin (AY859055), used as a normalizer.

Sestili et al. BMC Genomics 2011 12:122   doi:10.1186/1471-2164-12-122