Table 2

Selected list of Fusarium oxysporum f. sp. melonis (FOM) transcripts expressed in melon plants during infection.

ID

Bst-Mse

Length (bp)

Accessions

Annotation

Blast score

Category

ISPaVe1070

ISPaVe1018

ISPaVe1083


2 dpi

4 dpi

8 dpi

21 dpi

2 dpi

4 dpi

8 dpi

21 dpi

2 dpi

4 dpi

8 dpi

21 dpi


TDFs expressed only in planta


P1425

TG-CG

277

FOXT_01463

hypothetical protein similar to actin binding protein

3E-76

cell component

0

0

0

0

0

0

0

2

0

0

0

2

P1443

TA-CG

190

FOXG_00066

dynamin-2

6E-76

cell component

0

0

0

0

0

0

0

1

0

0

0

1

P406

CC-TT

479

FOXT_05654

hypothetical protein similar to 1,4-beta-glucanase

7E-124

cell wall

0

0

0

0

0

0

0

2

0

0

0

1

P998

CT-TG

174

FOXT_00113

chitin synthase 4

3E-52

cell wall

0

0

0

0

0

0

0

1

0

0

0

1

P1261

TG-AT

209

AF080485

Fusarium oxysporum f. sp. lycopersici pectate lyase

2E-87

cell wall-degrading

0

0

0

0

0

0

0

1

0

0

0

2

P553

CT-GT

235

FOXG_09638

endo-1,4-beta-xylanase 2 precursor

7E-101

cell wall-degrading

0

0

0

1

0

0

0

0

0

0

0

0

P1362

TC-GC

377

FOXT_13415

endo-1,4-beta-xylanase

9E-145

cell wall-degrading

0

0

0

0

0

0

0

2

0

0

0

1

P32

TT-AA

148

FOXG_06061

squalene synthetase

1E-42

metabolism

0

0

0

0

0

0

0

1

0

0

0

1

P675

CG-TT

259

FOXT_08412

protein similar to phosphoserine phosphatase

1E-114

signal transduction

0

0

0

0

0

0

0

1

0

0

0

1

P1473

TG-AG

224

FOXT_00565

hypothetical protein similar to calnexin

4E-102

signal transduction

0

0

0

0

0

0

0

1

0

0

0

1

P842

CT-AA

382

FOXG_03102

hypothetical protein similar to MADS box protein

4E-131

signal transduction

0

0

1

1

0

1

1

2

0

1

1

2

P1331

TC-CG

165

FVET_00351

hypothetical protein similar to vps28 protein

3E-24

transport

0

1

0

0

0

0

0

1

0

0

0

1

P888

TC-TA

449

FOXT_11289

plasma membrane ATPase

1E-178

transport

0

0

0

0

0

0

0

1

0

0

0

1

P1014

TT-GA

140

FOXT_12267

hypothetical protein similar to hexose transporter

8E-25

transport

0

0

0

0

0

0

0

2

0

0

0

1

P1066

CC-GC

380

FOXT_11113

mitochondrial phosphate carrier protein

1E-177

transport

0

0

0

0

0

0

0

1

0

0

0

1

P1232

CA-AT

341

FOXT_10521

mitochondrial import inner membrane translocase

1E-63

transport

0

0

0

0

0

0

0

1

0

0

0

1

P819

TC-AC

359

FOXG_09125

hypothetical protein similar to avenacinase

6E-117

virulence

0

0

0

0

0

0

0

3

0

0

0

1

P1258

TG-AT

468

FOXG_09782

siderophore iron transporter mirB

0

virulence

0

0

0

0

0

0

0

1

0

0

0

1

P1482

TC-TC

187

FOXT_03354

hypothetical protein similar to Fum16p

3E-81

virulence

0

0

0

0

0

0

0

2

0

0

0

2


TDFs expressed both in planta and in vitro


P417

CC-TT

168

FOXT_11103

hypothetical protein similar to sterol esterase precursor

6E-57

lipid metabolism

0

0

0

0

0

0

0

2

0

0

0

1

P292

TT-CG

180

FOXT_00536

UTP-glucose-1-phosphate uridylyltransferase

1E-77

metabolism

0

0

0

0

0

0

0

2

0

0

2

2

P305

CG-CA

199

FOXG_09447

pyruvate dehydrogenase E1 component subunit alpha

2E-88

metabolism

0

0

0

0

0

0

0

2

0

0

0

1

P344

CG-CC

363

FOXG_11520

NAD-specific glutamate dehydrogenase

1E-103

metabolism

0

0

0

0

0

0

0

3

0

0

0

2

P1211

CA-GC

159

FOXG_10419

malate dehydrogenase, mitochondrial precursor

1,00E-67

metabolism

0

0

0

0

0

0

0

2

0

0

0

2

P1333

TC-CG

140

FOXT_11398

UDP-glucose 6-dehydrogenase

4,00E-58

metabolism

0

0

0

0

0

0

0

3

0

0

0

3

P1486

CC-CA

226

FOXT_07937

glutathione reductase

1E-89

metabolism

0

0

0

0

0

0

0

1

0

0

0

1

P1280

TG-TC

358

FOXT_00266

hypothetical protein similar to multiubiquitin

1E-60

protein metabolism

0

0

0

0

0

0

0

1

0

0

0

1

P201

TA-TA

443

FOXG_03710

hypothetical protein similar to proteasome regulatory

0

protein metabolism

0

0

0

0

0

0

0

1

0

0

0

1

P1398

TA-GG

286

FOXT_00231

proteasome component C5

2E-139

protein metabolism

0

0

0

0

0

0

0

1

0

0

0

1

P304

CG-CA

215

FOXT_10713

hypothetical protein similar to Ca2+ ATPase

4E-68

signal transduction

0

0

0

0

0

0

0

1

0

0

0

1

P960

CT-TT

187

FOXG_05517

cAMP-dependent protein kinase regulatory subunit

6E-79

signal transduction

0

0

0

0

0

0

0

1

0

0

0

1

P469

CT-CA

294

FOXT_12260

peroxidase/catalase 2

7E-148

stress response

0

0

0

0

0

0

0

2

0

0

0

2

P1134

CG-GG

352

FOXT_01866

sorting nexin-41

0

transport

0

0

0

0

0

0

0

1

0

0

0

1

P464

CC-TG

136

FOXT_09879

hypothetical protein similar to neutral amino acid permease

8E-40

transport

0

0

0

0

0

0

0

2

0

0

0

1

P611

CT-GC

284

FOXG_09522

hypothetical protein similar to metalloreductase

1E-139

transport

0

0

0

0

0

0

0

3

0

0

0

3

P1293

TC-CA

396

FVET_08954

hypothetical protein similar to vacuolar sorting protein 35

0

transport

0

0

0

0

0

0

0

1

0

0

0

1

P365

CG-CG

426

FOXT_12915

arginase

0

virulence

0

0

0

0

0

0

0

1

0

0

0

1

P610

CT-GC

291

FOXT_05960

hypothetical protein similar to peroxisomal PEX11

1E-136

virulence

0

0

0

0

0

0

0

2

0

0

0

1


Selected FOM transcripts identified in infected melon plants, either specifically in planta, or both in planta and in vitro. Identification numbers (ID) correspond to progressive numbering of bands in the gels. The primer combination used to visualize each band (Bst-Mse column) and the corresponding length of the fragment are reported, along with the accession number in the database from which the sequence was retrieved, its annotation, the corresponding blast score and the functional category to which each transcript was assigned. In addition, the table reports the expression profile of each transcript, in each interaction and at each time point, estimated according to band intensity with a score from 1 to 3.

Sestili et al. BMC Genomics 2011 12:122   doi:10.1186/1471-2164-12-122

Open Data