Table 1 |
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Selected list of melon transcripts modulated after inoculating Fusarium oxysporum f. sp. melonis (FOM) on Charentais Fom-2 melon plants, representative of each Cluster. |
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|
ID |
Bst-Mse |
Length (bp) |
Melon Unigene 4.0 |
Uniprot |
Annotation |
Blast score |
Category |
ISPaVe1070 |
ISPaVe1018 |
ISPaVe1083 |
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|
|
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|
2 |
4 |
8 |
21 |
2 |
4 |
8 |
21 |
2 |
4 |
8 |
21 |
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|
CLUSTER A: TDFs specifically modulated in the incompatible interaction |
dpi |
dpi |
dpi |
dpi |
dpi |
dpi |
dpi |
dpi |
dpi |
dpi |
dpi |
dpi |
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|
|
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|
P552 |
CT-GT |
193 |
MU45835 |
P48351 |
Catalase isozyme 3 |
2E-80 |
Defence response |
0 |
0 |
1 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
P355 |
CG-CC |
153 |
MU54667 |
-- |
putative calmodulin-related protein |
3E-41 |
Signal transduction |
0 |
1 |
1 |
1 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
P1257 |
TG-AA |
119 |
MU54228 |
-- |
Protein translocase SEC61 complex gamma subunit |
4E-40 |
Transport |
0 |
-1 |
0 |
1 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
|
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|
CLUSTER B: TDFs specifically modulated in the compatible interaction at all time points |
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|
|
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|
P431 |
CC-TC |
254 |
-- |
Q69LA6 |
Probable pyridoxal biosynthesis protein PDX1.1. |
2E-28 |
Metabolism |
0 |
0 |
0 |
0 |
1 |
1 |
-1 |
-2 |
1 |
1 |
-1 |
-1 |
|
P75 |
TT-TA |
248 |
MU54623 |
-- |
Transmembrane CLPTM1 family protein |
7E-56 |
Signal transduction |
0 |
0 |
0 |
0 |
-1 |
-1 |
1 |
1 |
-1 |
-1 |
1 |
1 |
|
|
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|
CLUSTER C: TDFs specifically modulated in the compatible interaction, only at late time points (8 and 21 dpi) |
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|
|
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|
P554 |
CT-GT |
210 |
MU45886 |
P09918 |
13S-lipoxygenase |
2E-27 |
Defence response |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
2 |
0 |
0 |
0 |
3 |
|
P767 |
CA-CG |
115 |
MU47701 |
Q39799 |
Endochitinase 1 precursor |
2E-11 |
Defence response |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
2 |
0 |
0 |
1 |
2 |
|
P1190 |
CA-GT |
402 |
MU45840 |
-- |
type I proteinase inhibitor-like protein |
1E-123 |
Defence response |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
0 |
0 |
0 |
1 |
|
P763 |
CA-CC |
167 |
-- |
Q8S0S6 |
Gibberellin 2-oxidase |
3E-12 |
Metabolism |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
0 |
0 |
0 |
1 |
|
P962 |
CT-TT |
175 |
MU48285 |
-- |
12-oxophytodienoate reductase |
3E-27 |
Metabolism |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
2 |
0 |
0 |
1 |
2 |
|
P257 |
TT-CA |
354 |
MU52195 |
Q75GK0 |
IAA type protein |
E+00 |
Response to stimulus |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
2 |
0 |
0 |
1 |
2 |
|
P1011 |
TT-GA |
118 |
-- |
Q9LSQ4 |
Indole-3-acetic acid-amido synthetase GH3.6 |
2E-16 |
Response to stimulus |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
0 |
0 |
0 |
1 |
|
P1236 |
CA-AT |
53 |
MU51174 |
-- |
xanthine dehydrogenase-like protein |
5E-19 |
Response to stimulus |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
2 |
0 |
0 |
0 |
2 |
|
P1409 |
TG-CA |
305 |
-- |
O24174 |
Betaine aldehyde dehydrogenase (BADH). |
2E-18 |
Response to stimulus |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
0 |
0 |
0 |
1 |
|
P1430 |
TA-CC |
422 |
-- |
Q9S795 |
Betaine aldehyde dehydrogenase 1, chloroplast precursor (BADH) |
4E-23 |
Response to stimulus |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
0 |
0 |
0 |
1 |
|
P1418 |
TG-CC |
378 |
MU65227 |
B9RFG8 |
calmodulin-binding protein, putative, expressed |
5E-113 |
Signal transduction |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
0 |
0 |
0 |
1 |
|
P1479 |
TC-TC |
278 |
MU45785 |
P93087 |
Calmodulin (CaM) |
3E-129 |
Signal transduction |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
0 |
0 |
0 |
1 |
|
|
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|
CLUSTER D: TDFs repressed or induced at different stages in both the compatible and incompatible interactions |
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|
|
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|
P200 |
TA-TA |
445 |
MU48195 |
Q08506 |
ACC oxidase |
E+00 |
Defence response |
1 |
2 |
1 |
0 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
|
P413 |
CC-TT |
214 |
-- |
B9R992 |
ACC oxidase 1 |
2E-09 |
Defence response |
2 |
-1 |
-1 |
0 |
1 |
-1 |
-1 |
-2 |
1 |
-1 |
-1 |
-2 |
|
P449 |
CC-TG |
155 |
-- |
Q43419 |
ACC oxidase. |
7E-17 |
Defence response |
1 |
-1 |
0 |
-1 |
0 |
-1 |
0 |
0 |
1 |
0 |
0 |
0 |
|
P640 |
CG-AC |
325 |
-- |
Q40778 |
Allene oxide synthase |
3E-24 |
Defence response |
0 |
-1 |
1 |
2 |
-1 |
0 |
1 |
2 |
0 |
0 |
1 |
1 |
|
P870 |
CT-AG |
407 |
-- |
Q8H1S4 |
ACC oxidase homolog 4 |
3E-26 |
Defence response |
0 |
0 |
0 |
-1 |
0 |
0 |
0 |
1 |
0 |
0 |
0 |
1 |
|
P1109 |
CG-GT |
206 |
MU47271 |
-- |
Catalase isozyme 1 |
2E-92 |
Defence response |
0 |
0 |
0 |
-1 |
0 |
0 |
0 |
1 |
0 |
0 |
0 |
1 |
|
P285 |
TT-CC |
144 |
MU45886 |
B7FDE5 |
13S-lipoxygenase |
3E-29 |
Defence response |
-1 |
-1 |
0 |
1 |
-1 |
-1 |
1 |
0 |
-1 |
-1 |
1 |
1 |
|
P871 |
CT-AG |
390 |
-- |
Q9SFB5 |
Serine carboxypeptidase-like 27 precursor |
7E-56 |
Defence response |
-1 |
-1 |
-1 |
1 |
-1 |
-1 |
-1 |
-1 |
0 |
-1 |
-1 |
-1 |
|
P1327 |
TC-CG |
214 |
MU47122 |
Q9SQX6 |
Peptidase S10, serine carboxypeptidase |
5E-81 |
Defence response |
0 |
1 |
0 |
0 |
0 |
0 |
0 |
1 |
0 |
0 |
0 |
1 |
|
P534 |
CT-GA |
147 |
-- |
A7NSX3 |
Endo 1,4 beta glucanase |
1E-09 |
Defence response |
0 |
-1 |
-1 |
0 |
-1 |
-1 |
-1 |
-2 |
0 |
0 |
0 |
-1 |
|
P155 |
TG-GG |
172 |
MU55410 |
-- |
aspartokinase-homoserine dehydrogenase |
2E-70 |
Metabolism |
0 |
1 |
0 |
1 |
0 |
0 |
-1 |
0 |
0 |
0 |
-1 |
0 |
|
P273 |
TT-CT |
83 |
MU45201 |
-- |
HMG-CoA reductase |
1E-05 |
Metabolism |
0 |
0 |
0 |
-1 |
0 |
1 |
1 |
3 |
0 |
1 |
1 |
3 |
|
P517 |
CT-CG |
134 |
-- |
P68173 |
Adenosylhomocysteinase (S-adenosyl-L-homocysteine hydrolase) |
2E-13 |
Metabolism |
0 |
-1 |
-1 |
-1 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
-1 |
|
P996b |
CT-TG |
151 |
-- |
Q9SP37 |
Adenosylhomocysteise |
8E-15 |
Metabolism |
0 |
-1 |
-1 |
-1 |
0 |
0 |
0 |
-1 |
0 |
0 |
0 |
-1 |
|
P1336 |
TC-GA |
332 |
-- |
Q39110 |
Gibberellin 20 oxidase 1 |
2E-45 |
Metabolism |
1 |
0 |
0 |
1 |
0 |
0 |
0 |
-1 |
0 |
0 |
0 |
-1 |
|
P633 |
CG-AA |
190 |
-- |
B9GL14 |
Trehalase 1 GMTRE1 |
3E-19 |
Response to stimulus |
-1 |
0 |
0 |
0 |
0 |
0 |
1 |
2 |
0 |
0 |
1 |
2 |
|
P86 |
TT-TT |
167 |
MU58530 |
-- |
NDR1-like protein |
2E-51 |
Signal transduction |
1 |
1 |
0 |
0 |
0 |
0 |
1 |
0 |
0 |
0 |
1 |
0 |
|
P204 |
TA-TA |
384 |
MU46993 |
P43187 |
Ca2+-binding protein |
0E+00 |
Signal transduction |
0 |
2 |
1 |
2 |
0 |
0 |
1 |
2 |
0 |
0 |
1 |
2 |
|
P251 |
TA-CA |
64 |
MU52101 |
-- |
protein kinase family protein |
2E-24 |
Signal transduction |
0 |
1 |
1 |
1 |
-1 |
0 |
0 |
-1 |
1 |
1 |
-1 |
-1 |
|
P873 |
CT-AG |
360 |
-- |
Q9ZTX8 |
Auxin response factor 6. |
2E-19 |
Signal transduction |
0 |
-1 |
0 |
1 |
0 |
0 |
-1 |
-1 |
0 |
0 |
-1 |
-1 |
|
P1370 |
TC-GC |
222 |
MU50486 |
Q9FKW4 |
calcium-dependent protein kinase CDPK1444 |
1E-112 |
Signal transduction |
0 |
2 |
1 |
1 |
0 |
1 |
1 |
1 |
1 |
1 |
1 |
0 |
|
P1393 |
TA-GC |
247 |
MU51221 |
-- |
calcium-dependent calmodulin-independent protein kinase CDPK |
3E-18 |
Signal transduction |
0 |
1 |
0 |
0 |
0 |
1 |
1 |
1 |
0 |
1 |
1 |
1 |
|
P1478 |
TC-TC |
269 |
-- |
Q9LFL1 |
Receptor protein kinase-like protein |
2E-20 |
Signal transduction |
0 |
2 |
1 |
1 |
0 |
0 |
1 |
2 |
0 |
0 |
1 |
2 |
|
P1155 |
CC-AA |
108 |
-- |
Q37145 |
Calcium-transporting ATPase 1, chloroplast precursor |
7E-10 |
Signal transduction |
0 |
1 |
1 |
2 |
0 |
0 |
1 |
0 |
0 |
0 |
0 |
0 |
|
P57 |
TT-AC |
237 |
-- |
Q8H0E1 |
AUX1-like auxin transport protein |
4E-11 |
Transport |
1 |
1 |
2 |
2 |
0 |
0 |
-1 |
-1 |
0 |
0 |
0 |
-1 |
|
P1100 |
CG-GA |
105 |
MU54228 |
Q9SW34 |
Protein translocase SEC61 complex gamma subunit |
2E-44 |
Transport |
0 |
2 |
0 |
1 |
-2 |
-1 |
-1 |
-2 |
-1 |
-1 |
0 |
-1 |
|
|
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|
This selection of representative genes modulated by infection with avirulent (ISPaVe1070 race1) or virulent (ISPaVe1018 and ISPaVe1083 race 1,2) strains of FOM is divided into clusters as defined in the results section. Identification numbers (ID) correspond to progressive numbering of bands in the gels. The primer combination used to visualize each band (Bst-Mse column) and the corresponding length of the fragment are reported, along with the accession number in the database from which the sequence was retrieved, its annotation, the corresponding BLAST score and the functional category to which each transcript was assigned. In addition, the table shows the expression profile of each transcript, in each interaction and at each time point, estimated on the basis of the band intensity with a score from -3 to 3, in comparison to the corresponding band in the uninfected controls. |
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|
Sestili et al. BMC Genomics 2011 12:122 doi:10.1186/1471-2164-12-122 |
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