Table 1

Selected list of melon transcripts modulated after inoculating Fusarium oxysporum f. sp. melonis (FOM) on Charentais Fom-2 melon plants, representative of each Cluster.

ID

Bst-Mse

Length (bp)

Melon Unigene 4.0

Uniprot

Annotation

Blast score

Category

ISPaVe1070

ISPaVe1018

ISPaVe1083


2

4

8

21

2

4

8

21

2

4

8

21

CLUSTER A: TDFs specifically modulated in the incompatible interaction

dpi

dpi

dpi

dpi

dpi

dpi

dpi

dpi

dpi

dpi

dpi

dpi


P552

CT-GT

193

MU45835

P48351

Catalase isozyme 3

2E-80

Defence response

0

0

1

0

0

0

0

0

0

0

0

0

P355

CG-CC

153

MU54667

--

putative calmodulin-related protein

3E-41

Signal transduction

0

1

1

1

0

0

0

0

0

0

0

0

P1257

TG-AA

119

MU54228

--

Protein translocase SEC61 complex gamma subunit

4E-40

Transport

0

-1

0

1

0

0

0

0

0

0

0

0


CLUSTER B: TDFs specifically modulated in the compatible interaction at all time points


P431

CC-TC

254

--

Q69LA6

Probable pyridoxal biosynthesis protein PDX1.1.

2E-28

Metabolism

0

0

0

0

1

1

-1

-2

1

1

-1

-1

P75

TT-TA

248

MU54623

--

Transmembrane CLPTM1 family protein

7E-56

Signal transduction

0

0

0

0

-1

-1

1

1

-1

-1

1

1


CLUSTER C: TDFs specifically modulated in the compatible interaction, only at late time points (8 and 21 dpi)


P554

CT-GT

210

MU45886

P09918

13S-lipoxygenase

2E-27

Defence response

0

0

0

0

0

0

0

2

0

0

0

3

P767

CA-CG

115

MU47701

Q39799

Endochitinase 1 precursor

2E-11

Defence response

0

0

0

0

0

0

1

2

0

0

1

2

P1190

CA-GT

402

MU45840

--

type I proteinase inhibitor-like protein

1E-123

Defence response

0

0

0

0

0

0

0

1

0

0

0

1

P763

CA-CC

167

--

Q8S0S6

Gibberellin 2-oxidase

3E-12

Metabolism

0

0

0

0

0

0

0

1

0

0

0

1

P962

CT-TT

175

MU48285

--

12-oxophytodienoate reductase

3E-27

Metabolism

0

0

0

0

0

0

1

2

0

0

1

2

P257

TT-CA

354

MU52195

Q75GK0

IAA type protein

E+00

Response to stimulus

0

0

0

0

0

0

1

2

0

0

1

2

P1011

TT-GA

118

--

Q9LSQ4

Indole-3-acetic acid-amido synthetase GH3.6

2E-16

Response to stimulus

0

0

0

0

0

0

0

1

0

0

0

1

P1236

CA-AT

53

MU51174

--

xanthine dehydrogenase-like protein

5E-19

Response to stimulus

0

0

0

0

0

0

0

2

0

0

0

2

P1409

TG-CA

305

--

O24174

Betaine aldehyde dehydrogenase (BADH).

2E-18

Response to stimulus

0

0

0

0

0

0

0

1

0

0

0

1

P1430

TA-CC

422

--

Q9S795

Betaine aldehyde dehydrogenase 1, chloroplast precursor (BADH)

4E-23

Response to stimulus

0

0

0

0

0

0

0

1

0

0

0

1

P1418

TG-CC

378

MU65227

B9RFG8

calmodulin-binding protein, putative, expressed

5E-113

Signal transduction

0

0

0

0

0

0

0

1

0

0

0

1

P1479

TC-TC

278

MU45785

P93087

Calmodulin (CaM)

3E-129

Signal transduction

0

0

0

0

0

0

0

1

0

0

0

1


CLUSTER D: TDFs repressed or induced at different stages in both the compatible and incompatible interactions


P200

TA-TA

445

MU48195

Q08506

ACC oxidase

E+00

Defence response

1

2

1

0

1

1

1

1

1

1

1

1

P413

CC-TT

214

--

B9R992

ACC oxidase 1

2E-09

Defence response

2

-1

-1

0

1

-1

-1

-2

1

-1

-1

-2

P449

CC-TG

155

--

Q43419

ACC oxidase.

7E-17

Defence response

1

-1

0

-1

0

-1

0

0

1

0

0

0

P640

CG-AC

325

--

Q40778

Allene oxide synthase

3E-24

Defence response

0

-1

1

2

-1

0

1

2

0

0

1

1

P870

CT-AG

407

--

Q8H1S4

ACC oxidase homolog 4

3E-26

Defence response

0

0

0

-1

0

0

0

1

0

0

0

1

P1109

CG-GT

206

MU47271

--

Catalase isozyme 1

2E-92

Defence response

0

0

0

-1

0

0

0

1

0

0

0

1

P285

TT-CC

144

MU45886

B7FDE5

13S-lipoxygenase

3E-29

Defence response

-1

-1

0

1

-1

-1

1

0

-1

-1

1

1

P871

CT-AG

390

--

Q9SFB5

Serine carboxypeptidase-like 27 precursor

7E-56

Defence response

-1

-1

-1

1

-1

-1

-1

-1

0

-1

-1

-1

P1327

TC-CG

214

MU47122

Q9SQX6

Peptidase S10, serine carboxypeptidase

5E-81

Defence response

0

1

0

0

0

0

0

1

0

0

0

1

P534

CT-GA

147

--

A7NSX3

Endo 1,4 beta glucanase

1E-09

Defence response

0

-1

-1

0

-1

-1

-1

-2

0

0

0

-1

P155

TG-GG

172

MU55410

--

aspartokinase-homoserine dehydrogenase

2E-70

Metabolism

0

1

0

1

0

0

-1

0

0

0

-1

0

P273

TT-CT

83

MU45201

--

HMG-CoA reductase

1E-05

Metabolism

0

0

0

-1

0

1

1

3

0

1

1

3

P517

CT-CG

134

--

P68173

Adenosylhomocysteinase (S-adenosyl-L-homocysteine hydrolase)

2E-13

Metabolism

0

-1

-1

-1

0

0

0

0

0

0

0

-1

P996b

CT-TG

151

--

Q9SP37

Adenosylhomocysteise

8E-15

Metabolism

0

-1

-1

-1

0

0

0

-1

0

0

0

-1

P1336

TC-GA

332

--

Q39110

Gibberellin 20 oxidase 1

2E-45

Metabolism

1

0

0

1

0

0

0

-1

0

0

0

-1

P633

CG-AA

190

--

B9GL14

Trehalase 1 GMTRE1

3E-19

Response to stimulus

-1

0

0

0

0

0

1

2

0

0

1

2

P86

TT-TT

167

MU58530

--

NDR1-like protein

2E-51

Signal transduction

1

1

0

0

0

0

1

0

0

0

1

0

P204

TA-TA

384

MU46993

P43187

Ca2+-binding protein

0E+00

Signal transduction

0

2

1

2

0

0

1

2

0

0

1

2

P251

TA-CA

64

MU52101

--

protein kinase family protein

2E-24

Signal transduction

0

1

1

1

-1

0

0

-1

1

1

-1

-1

P873

CT-AG

360

--

Q9ZTX8

Auxin response factor 6.

2E-19

Signal transduction

0

-1

0

1

0

0

-1

-1

0

0

-1

-1

P1370

TC-GC

222

MU50486

Q9FKW4

calcium-dependent protein kinase CDPK1444

1E-112

Signal transduction

0

2

1

1

0

1

1

1

1

1

1

0

P1393

TA-GC

247

MU51221

--

calcium-dependent calmodulin-independent protein kinase CDPK

3E-18

Signal transduction

0

1

0

0

0

1

1

1

0

1

1

1

P1478

TC-TC

269

--

Q9LFL1

Receptor protein kinase-like protein

2E-20

Signal transduction

0

2

1

1

0

0

1

2

0

0

1

2

P1155

CC-AA

108

--

Q37145

Calcium-transporting ATPase 1, chloroplast precursor

7E-10

Signal transduction

0

1

1

2

0

0

1

0

0

0

0

0

P57

TT-AC

237

--

Q8H0E1

AUX1-like auxin transport protein

4E-11

Transport

1

1

2

2

0

0

-1

-1

0

0

0

-1

P1100

CG-GA

105

MU54228

Q9SW34

Protein translocase SEC61 complex gamma subunit

2E-44

Transport

0

2

0

1

-2

-1

-1

-2

-1

-1

0

-1


This selection of representative genes modulated by infection with avirulent (ISPaVe1070 race1) or virulent (ISPaVe1018 and ISPaVe1083 race 1,2) strains of FOM is divided into clusters as defined in the results section. Identification numbers (ID) correspond to progressive numbering of bands in the gels. The primer combination used to visualize each band (Bst-Mse column) and the corresponding length of the fragment are reported, along with the accession number in the database from which the sequence was retrieved, its annotation, the corresponding BLAST score and the functional category to which each transcript was assigned. In addition, the table shows the expression profile of each transcript, in each interaction and at each time point, estimated on the basis of the band intensity with a score from -3 to 3, in comparison to the corresponding band in the uninfected controls.

Sestili et al. BMC Genomics 2011 12:122   doi:10.1186/1471-2164-12-122

Open Data