Open Access Research article

Distinct colonization patterns and cDNA-AFLP transcriptome profiles in compatible and incompatible interactions between melon and different races of Fusarium oxysporum f. sp. melonis

Sara Sestili1, Annalisa Polverari2*, Laura Luongo3, Alberto Ferrarini2, Michele Scotton4, Jamshaid Hussain2, Massimo Delledonne2, Nadia Ficcadenti1 and Alessandra Belisario3*

Author Affiliations

1 Agricultural Research Council (CRA), Research Unit for Vegetable Crop in Central Areas, Via Salaria 1, 63030 Monsampolo del Tronto (AP), Italy

2 Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134 Verona, Italy

3 Agricultural Research Council (CRA), Plant Pathology Research Center, Via C.G. Bertero 22, 00156 Roma, Italy

4 Department of Environmental Agronomy and Crop Production, University of Padova, Viale dell'Università 16, 35020 Legnaro, Padova, Italy

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BMC Genomics 2011, 12:122  doi:10.1186/1471-2164-12-122

Published: 21 February 2011

Additional files

Additional file 1:

Complete list of melon genes modulated by infection with avirulent (ISPaVe1070 race1) or virulent (ISPaVe1018 and ISPaVe1083 strains of race 1,2) strains of Fusarium oxysporum f. sp. melonis, organized by clusters as defined in the results section. Identification numbers (ID) correspond to the progressive numbering of bands in the gels. The primer combination used to visualize each band (Bst-Mse column) and the corresponding length of the fragment are reported, along with the accession number retrieved from the Melon Unigene or Uniprot database, the annotation, the corresponding blast score, and the functional category to which each transcript was assigned. In addition, the file reports the expression profile of each transcript, estimated on the basis of the band intensity with a score ranging from -3 to 3, in comparison to the corresponding band in the mock-inoculated controls.

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Additional file 2:

Complete list of Fusarium oxysporum f. sp. melonis (FOM) transcripts identified in infected melon plants, either specifically in planta, or both in planta and in vitro. Identification numbers (ID) correspond to the progressive numbering of bands in the gels. The primer combination used to visualize each band (Bst-Mse column) and the corresponding length of the fragment are reported, along with the accession number retrieved from the database, the annotation, the corresponding blast score, and the functional category to which each transcript was assigned. In addition, the file reports the expression profile of each transcript, in each interaction and at each time point, estimated on the basis of the band intensity with a score ranging from 1 to 3.

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Additional file 3:

List of the core 54 melon genes, which remain modulated in a coherent way (induced or repressed) throughout the experiment (from 4 dpi onwards) in the incompatible interaction between melon and the FOM race 1 strain ISPaVe1070.

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Additional file 4:

Complete list of Fusarium oxysporum f. sp. melonis (FOM) transcripts identified by cDNA-AFLP analysis from fungal samples of colonies grown in vitro and selected for sequencing. TDFs were chosen on the basis of their differential abundance in the different FOM strains. Identification numbers (ID) correspond to the progressive numbering of bands in the gels. The primer combination used to visualize each band (Bst-Mse column) and the corresponding length of the fragment are reported, along with the accession number retrieved from the database and the corresponding blast score and annotation. In addition, the file reports the presence (+) or absence (-) of expression of each transcript for the 3 strains of FOM.

Format: XLS Size: 35KB Download file

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Additional file 5:

List of primers used in Real time RT-PCR analysis. Identification numbers (ID) correspond to the progressive numbering of bands in the gels. The accession codes for each gene represent the corresponding database in which homology was identified, MU = Melon Unigene, FOX = Fusarium Comparative Database.

Format: XLS Size: 25KB Download file

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