Limit of detection of ERCC RNA standards. Incidence of failed PCR reactions on ABI 7900HT system or BioMark using cDNA or pre-amplified cDNA as the template. Mean percentage of failed PCR reactions ± SD are displayed based on data from 8 different RNA standards from 3 independent qPCR experiments across a range from 1 to 106 copies ERCC standard per ng total RNA.
Devonshire et al. BMC Genomics 2011 12:118 doi:10.1186/1471-2164-12-118