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Open Access Methodology article

Applicability of RNA standards for evaluating RT-qPCR assays and platforms

Alison S Devonshire, Ramnath Elaswarapu* and Carole A Foy

Author Affiliations

LGC Limited, Queens Road, Teddington, Middlesex, TW11 0LY, UK

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BMC Genomics 2011, 12:118  doi:10.1186/1471-2164-12-118

Published: 18 February 2011

Additional files

Additional file 1:

Taqman assays for ERCC RNA standards. Microsoft Word file detailing the sequences of primers and probes used for qPCR assays.

Format: DOC Size: 47KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 2:

MIQE Additional Information. Microsoft Excel file containing further information on RNA preparation, purity, PCR efficiency and negative control data complying with the MIQE guidelines.

Format: XLS Size: 2.5MB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 3:

MIQE Checklist. Checklist in Microsoft Word format detailing information complying with MIQE guidelines.

Format: DOCX Size: 22KB Download file

Open Data