Single-cell tracking identifies different profiles of transcriptional activation. 293T cells transfected with p(GRE)x3-AdMLP-VC5 were induced with 1 × 10-7M dexamethasone and tracked over 24 hours. (A, C) Tracks of cells inducing (A) and remaining unchanged (C) over 24 hours. Each cell track is represented by a line where each dot represents a different timepoint and the color describes the time of induction from 0 (blue) to 24 hours (red). The lower teal line represents the mean of the starting population, while the upper teal line shows the mean + 3 standard deviations. Single-cell time-lapse images are shown for cells that have similar starting ECFP expression levels, but whose response to dexamethasone is either high (B) or remains unchanged over time (D).
Rajan et al. BMC Genomics 2011 12:115 doi:10.1186/1471-2164-12-115