Measurement of transcription using a dual-fluorophore vector. (A) Time-lapse images of (GRE)x3-AdMLP induction by dexamethasone in 293T cells using nuclear and cytoplasmic reporters. The first two rows represent the same spot transfected with the p(GRE)x3-AdMLP-VC5 vector and imaged in ECFP (top) and Venus (middle) channels. The induction profile using the Venus reporter is comparable to that of the cytoplasmic destabilized EGFP reporter, p(GRE)x3-AdMLP-d2EGFP, shown in the bottom row. Moreover, the use of the ECFP channel enables the tracking of transfected cells before induction has taken place. Scale bar = 100μm. (B) Map of the Venus-ECFP5 (VC5) plasmid.
Rajan et al. BMC Genomics 2011 12:115 doi:10.1186/1471-2164-12-115