Living Microarray method. (A) Platform Workflow. The Living Microarrays method allows for highly parallelized measurement of reporter gene expression at the single-cell level. Synthetic promoter cassettes are cloned into the VC5 reporter construct and spotted with transfection reagent onto glass slides. Transfected cell clusters are imaged iteratively using automated microscopy and analyzed to generate normalized single-cell measurements of transcriptional activity. (B) Whole chip view of a Living Microarray. HEK-293T cells were reverse-transfected at 600 spots with varying ratios of pEGFP-N3 and Lipofectamine2000 and fixed cells were imaged at 10 μm resolution using a microarray scanner. Maximal transfection conditions are indicated by the white box and shown at higher magnification. (C) Reverse transfection reporter microarrays using human, mouse and rat cell lines originating from various tissues transfected with pEGFP-N3. Scale bars = 350 μm.
Rajan et al. BMC Genomics 2011 12:115 doi:10.1186/1471-2164-12-115