Validation of the microarray data by quantitative real-time RT-PCR analysis of eight representative genes. The x-axis represents the genes and the y-axis shows the relative expression (-ΔCt values for quantitative real-time RT-PCR; Log(Sample signal, 10) for microarray). The number of biological replicates is three for both assays. R represents the Pearson correlation coefficient. The significance of differences for the expression between Chinese Taihu (TH) and Large White (LW) PMSG-hCG stimulated ovary follicles was calculated using two-tailed T-test. p value: left for microarray, and right for quantitative real-time RT-PCR (qRT-PCR).
Sun et al. BMC Genomics 2011 12:111 doi:10.1186/1471-2164-12-111