Table 1

Genes showing synthetic interaction with CCW12

ORF

Gene

Description of gene product

SGA

Fold change(a)

Interaction in other cell wall networks(b)

Published interaction (c)


Array

qRT-PCR

    Cell wall synthesis and regulation

YBL061C

CHS4

Activator of Chs3p (chitin synthase III), recruits Chs3p to the bud neck via interaction with Bni4p

ss

FKS1, SMI1, GAS1

-

YBR023C

CHS3

Chitin synthase III

ss

2.5

3.2 ± 0.7

FKS1, SMI1, GAS1

[27,30]

YDR245W

MNN10

Subunit of the Mannan polymerase II complex

sl

CHS3, CHS4, CHS5, CHS6, CHS7, SMI1

-

YHR030C

SLT2

MAP-kinase of the cell integrity pathway

sl

2.2

1.7 ± 0.1

BNI4, CHS3, CHS4, CHS5, CHS6, CHS7, FKS1, GAS1, SMI1

[31]

YHR142W

CHS7

Involved in chitin biosynthesis by regulating Chs3p export from the ER

ss

FKS1, SMI1, GAS1

[27,30,31]

YJL095W

BCK1

MAPKK-kinase of the cell integrity pathway

sl

2.5

2.7 ± 0.5

BNI4, CHS1, CHS3, CHS4, CHS5, CHS7, GAS1, SMI1

[31]

YLR113W

HOG1

Mitogen-activated protein kinase involved in osmoregulation

ss

-

YLR330W

CHS5

Involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane

sl

FKS1, SMI1, GAS1

[27,30]

YLR332W

MID2

Sensor for the PKC1-SLT2 cell wall integrity pathway

ss

FKS1, SMI1

[27,29,31]

    Cell polarity and vesicular transport

YBL007C

SLA1

Cortical actin patch assembly control protein, mutation affects endocytosis

sl

2.7

2.7 ± 0.1

CHS3, CHS4, CHS5, CHS7, FKS1, SMI1

[31]

YBL047C

EDE1

Cortical actin patch protein with a role in endocytosis

ss

2.6

2.7 ± 0.5

BNI4, CHS3, CHS4, CHS5, CHS7, FKS1

[31]

YCR034W

FEN1

Involved in fatty acids elongation

ss

FKS1

-

YDR129C

SAC6

Involved in the organization and maintenance of the actin cytoskeleton

sl

CHS3, CHS4, CHS5, CHS7

-

YJL179W

PFD1

Involved in the biogenesis of actin and of alpha- and gamma-tubulin

sl

2.5

1.7 ± 0.4

-

YLR370C

ARC18

Required for the motility and integrity of cortical actin patches

sl

CHS1, CHS3, CHS4, CHS5, CHS6

-

YNR051C

BRE5

Activator of Ubp3p that regulates COPII coat assembly

ss

BNI4, CHS3, CHS4, FKS1, SMI1

[31]

YOR035C

SHE4

Involved in cortical actin patch assembly and endocytosis

ss

CHS3, CHS4, CHS7, FKS1

[31]

    Other genes

YLR119W

SRN2

Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome

ss

-

YNL197C

WHI3

Regulates the critical cell size required for passage through Start

ss

-

YDR417C

Hypothetical protein, potential GPI anchor and secretion sequence

ss

-

YGR078C

PAC10

Polypeptide 3 of a Yeast Non-native Actin Binding Complex

sl

-


(a) Data correspond to the ratio of expression of ccw12 versus wt calculated as described in "Methods".

(b) [30,60]

(c) Published Genetic Interactions with CCW12.

Ragni et al. BMC Genomics 2011 12:107   doi:10.1186/1471-2164-12-107

Open Data