Budding and formation of the mating projection are affected in ccw12Δ cells. Wt (SEY6211) and ccw12Δ mutant (MEY12B) strains were treated with the mating pheromone α-factor as detailed in Methods. At time zero 20 μg/ml of α-factor were added. Budding index (A) and shmoo formation (B) was monitored over the time. Mean values of four independent experiments are shown. At least 200 cells were analysed at the indicated times.
Ragni et al. BMC Genomics 2011 12:107 doi:10.1186/1471-2164-12-107