Figure 4.

Activation of the CWI pathway in mutant ccw12Δ. Mutant ccw12Δ, slt2Δ, gas1Δ and BY4741 (isogenic wt) cells, were grown to mid-exponential phase, and cell extracts were analysed by Western blot using phospho-p44/p42 MAPK antibodies to detect dually phosphorylated Slt2p (mid and low panel, PP-Slt2p). Pfk1p was used as a loading control (upper panel, Pfk1p). PP-Slt2p and Pfk1p protein levels were quantified as described in Figure 1

Ragni et al. BMC Genomics 2011 12:107   doi:10.1186/1471-2164-12-107
Download authors' original image