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Open Access Highly Accessed Research article

Venom gland transcriptomes of two elapid snakes (Bungarus multicinctus and Naja atra) and evolution of toxin genes

Yu Jiang12, Yan Li3, Wenhui Lee4, Xun Xu1, Yue Zhang1, Ruoping Zhao1, Yun Zhang4* and Wen Wang1*

Author Affiliations

1 CAS-Max Planck Junior Research Group, State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China

2 Graduate University of Chinese Academy Sciences, Beijing 100039, China

3 College of Animal Science and Technology, Sichuan Agricultural University, Sichuan 625014, China

4 Biotoxin Units, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China

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BMC Genomics 2011, 12:1  doi:10.1186/1471-2164-12-1

Published: 3 January 2011

Additional files

Additional file 1:

The complete list of all EST clusters in B. multicinctus and N. atra, annotated by using BLAST program.

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Additional file 2:

Figure S1 and S2. Phylogenetic trees constructed, respectively, using Kunitz and lectins sequences with the maximum likelihood method in MEGA. Figure S3. Characterization of the snake BAC libraries. Sixteen randomly selected B. multicinctus and N. atra BAC clones were digested with Not I and separated by PFGE. Lane M: Low-range PFG marker. Table S1. The representative sequences used for estimation of the dN/dS ratios. Table S2. The detailed information of all 71 positive toxin BAC clones from the toxin probe screening. Table S3. The toxin ESTs used as hybridization probes to screen for venom genes.

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