Procedure of probe design for in vitro homology search array. For every genes stored in the public database for human and squid genes, we extracted 60 bp candidate probes at intervals of 50bp. Edit distances were calculated and the appropriate probes were selected according to the following criteria; single genes, more than 11 edit distance, most 3' probe. A total of 11,559 probes were designed on the array, including 7,987 human probes and 3,572 squid probes.
Ogura et al. BMC Genomics 2010 11(Suppl 4):S9 doi:10.1186/1471-2164-11-S4-S9