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## Figure 7.
32 transcripts is an optimum threshold for achieving similar cumulative frequency
distribution amongst replicates. KS statistics was applied iteratively to our biological replicates (red and black
lines), each graph depicts a gradual change in the threshold value. The KS test statistics
can be thought of as a cost function that we seek to minimize to ensure that the distributions
between the two replicates are similar. Applying such a strategy iteratively as we
change the threshold value gradually, we arrive at a point where both line converge
together indicating similar distributions. The point where this first occurs is designated
as the minimum threshold count value of biological significance.
Koh |