Different hES-MSC replicates show similar alignment peaks. Visualization of Seqmap mapping via UCSC genome browser reveals peaks along specific genomic regions that have large numbers of miRNA binding to these regions. In the top figure A Blue and Purple trend lines represent biological replicates of samples derived from intracellular environment. High degree of correlation is observed between the replicates as can be observed from the similarity in locations where peaks were found. The height of each such peak corresponds to the number of transcripts detected from deep sequencing. Each peak now represents genomic locations where a large number of specific transcripts bind to. The bottom figure B depicts the extra cellular sample transcripts that are aligned to the human genome using Seqmap. Peaks occur in similar region after mapping across the replicates. Each replicate is visualized with a different color and the salient feature reveals peaks from different sample aligning in similar locations.
Koh et al. BMC Genomics 2010 11(Suppl 1):S6 doi:10.1186/1471-2164-11-S1-S6