Additional Venn diagram analysis. A) Considerable overlap in top ranked genes from comparing of highly-expressed microarray and sequencing data for intracellular miRNAs. B) Overlap in top ranked genes from comparing of highly-expressed microarray and sequencing data for extracellular miRNAs is low. C) Top-level sequence data shows strong agreement between intra and extra cellular samples. Expression microarray for miRNAs were performed and compared with our deep sequencing data. Ranking of the top miRNAs by their abundance from both techniques was performed. Top hundred miRNAs for the intracellular samples were compared using kappa correlation analysis and a large degree of overlap between studied sets was observed. Similarly for the extra cellular samples, the top 50 miRNAs were compared. The degree of overlap between studied sets was less than that of the intracellular samples. A final comparison involves that of the common genes amongst replicates of the intracellular samples with the extra cellular samples. In this case we found that most of the genes of the extracellular microRNA form a common subset of the genes of the intracellular miRNA.
Koh et al. BMC Genomics 2010 11(Suppl 1):S6 doi:10.1186/1471-2164-11-S1-S6