Different stages of processing microRNA sequences to gain biological insight. Massive sequencing data can be overwhelming to examine, with each stage of processing choices are made to direct analysis towards biological significance and understanding. Initial processing stages emphases on visualization of datasets in different forms such as annotations and alignment in the genome. This is to allow for quick assessment of salient features. Following this are more advanced analysis exploiting existing tools for examining distributions and networks topology to conjure biological hypothesis for verification. The entire workflow can then be thought as a funneling process towards biologically interesting interactions for verification in our hES-MSC cell line.
Koh et al. BMC Genomics 2010 11(Suppl 1):S6 doi:10.1186/1471-2164-11-S1-S6