Research article
Development of a EST dataset and characterization of EST-SSRs in a traditional Chinese medicinal plant, Epimedium sagittatum (Sieb. Et Zucc.) Maxim
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* Corresponding author: Ying Wang yingwang@wbgcas.cn
- † Equal contributors
1 Key Laboratory of Pant Germplasm Enhancement and Speciality Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei 430074, China
2 The Graduate School, Chinese Academy of Sciences, Beijing, 100039, China
3 Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY 14853, USA
4 USDA Robert W. Holley Center for Agriculture and Health, Ithaca, NY 14853, USA
5 Stephenson Research and Technology Center, University of Oklahoma, Norman, Oklahoma 73019, USA
BMC Genomics 2010, 11:94 doi:10.1186/1471-2164-11-94
Published: 8 February 2010Additional files
Additional file 1:
Table S1. Characterization of E. sagittatum ESTs before and after assembly
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Additional file 2:
Table S2. Characterization of 18 EST-SSR loci in 52 Epimedium species. Note: ※ indicated these primer pairs transfer in Epimedium species with a single band or null. EPS means expected product size for E. sagittatum. OPS means observed product size. ND means not determined.
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Additional file 3:
Table S3. Epimedium species used for detecting the transferability of EST-SSR. Note: E. koreanum1, E. koreanum2, E. koreanum3 indicated that the specimens were collected from Korea, China and Japan, respectively.
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Additional file 4:
Figure S1. Dendrogram representing the relationships observed in 52 Epimedium species based on 16 EST-SSR markers. V. hexandra was used as an outgroup and the distance bar was shown on the bottom of the tree. The number nearby the branch was bootstrap values and the bootstrap value lower than 30% were not shown.
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Additional file 5:
Figure S2. Correlation test between consensus annotation percentage and consensus length. Pearson test show that annotation percentage is positively related to consensus length with correlation coefficients 0.96 at the level of 0.01.
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