Figure 4.

Distribution of fragment sizes after shearing the circular and linear cosmid DNA. Aliquots of the sheared DNA samples used to construct the sequencing libraries were run on a 0.7% agarose gel alongside a 1 kb plus size marker. The gel was stained with SybrGold and scanned in a FluorImager. Molecular abundance was measured using an arbitrary value which was calculated by dividing the fluorescence intensity by molecular length. A) shows the resulting digital image. B) shows a graph of the size distributions for the three sheared DNA samples.

Schwartz and Farman BMC Genomics 2010 11:87   doi:10.1186/1471-2164-11-87
Download authors' original image