MicroRNAs show diverse and dynamic expression patterns in multiple tissues of Bombyx mori
1 The Key Sericultural Laboratory of Agricultural Ministry, College of Biotechnology, Southwest University, Chongqing 400715, PR China
2 Institute of Agricultural and Life Sciences, Chongqing University, Chongqing, 400030, PR China
BMC Genomics 2010, 11:85 doi:10.1186/1471-2164-11-85Published: 2 February 2010
Additional file 1:
Tissue- and organ-specific changes in miRNA expression. Normalized data of expressed miRNAs were submitted to SAM 3.0 for time-course analysis. miRNAs displaying downregulation or upregulation in all tissues during metamorphosis are presented in light blue. miRNAs displaying downregulation or upregulation in at least two tissues are depicted in light purple, while those downregulated or upregulated in only one tissue are colored gray. The miRNAs colored light yellow are upregulated in one tissue, but downregulated in another tissue. Only miRNAs up- or downregulated in at least one tissue are presented. Abbreviations: BW, body wall; SG, silk gland; MG, midgut; FB, fat body; reg, regulated; score (d), the T-statistic value; up, upregulated; down, downregulated.
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Additional file 2:
Microarray-based analyses of coordinate transcription and asymmetric transcription of miRNAs. Co-expression was usually observed for miRNAs derived from both arms of the same precursors, as well as for some miRNAs generated at either strand of the same locus. (A) and (B) Not all miRNAs from both arms of the precursors were coordinately expressed; (C) and (D) likewise, not all miRNAs from both strands of the same locus were coordinately accumulated. (A) Comparison of 3p/5p miRNA pairs in multiple tissues of fifth-instar day 3 larvae. (B) Comparison of 3p/5p miRNA pairs in the four tissues undergoing metamorphosis from larval to pupal stages. (C) Comparison of sense and antisense miRNAs in multiple tissues of fifth-instar day 3 larvae. (D) Comparison of sense and antisense miRNAs in the four tissues undergoing metamorphosis. Abbreviations: HD, head; BW, body wall; ASG, anterior silk gland; PSG, posterior silk gland; MG, midgut; FB, fat body; OV, ovary; TE, testis; HC, hemocyte; MT, malpighian tubule; f, female; m, male; 7d IL5, fifth-instar day 7 larvae; 0hr Sp, 0-hour spinning larvae; 0hr PPu, 0-hour prepupae; 1d Pu, day 1 pupae; 'a' and 'b' represent the average signals of each probe printed at three points on individual blocks.
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