Table 1

Selection of candidate reference genes from microarray data

Gene symbol

Definition

Mean

geNorm

NormFinder


RPL29

Ribosomal protein L29

14.04

1

1

RPL10a

Ribosomal protein L10A

12.49

2

2

LOC292640

Vps20-associated 1 homolog

10.87

3

3

LOC498143

Similar to ribosomal protein L15

13.71

4

4

LOC317275

Similar to ribosomal protein L7-like 1

11.88

7

5

RPS15

Ribosomal protein S15

12.97

5

6

ARBP

Acidic ribosomal phosphoprotein P0

14.27

6

7

RPL14

Ribosomal protein L14

13.89

9

8

EEF1A1

Eukaryotic translation elongation factor 1 alpha 1

14.17

8

9

RPS15A

Ribosomal protein S15a

13.93

10

10

RPL18

Ribosomal protein L18

13.58

11

11

REPS1 (P)

RalBP1 associated Eps domain containing protein (predicted)

10.73

12

12

LOC363720

chromatin modifying protein 2B

10.61

14

13

CNOT8

CCR4-NOT transcription complex, subunit 8

11.00

15

14

RTCD1

RNA terminal phosphate cyclase domain 1

10.48

17

15

RPL19

Ribosomal protein L19

13.74

13

16

NDUFB6 (P)

NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 6,

10.43

16

17

RPL9

Ribosomal protein L9

13.74

18

18

LOC499803

Similar to 40S ribosomal protein S3

13.76

19

19

RPL3

Ribosomal protein L3

14.03

20

20


ACTB

Actin, beta

13.85

GAPDH

Glyceraldehyde-3-phosphate dehydrogenase

12.88


Microarray analysis of the expression profiles of 21,910 genes in naïve PC12 cells and those treated with NGF or GDNF for 0.5 h and 72 h. Twenty candidate reference genes were selected based on pairwise comparison (geNorm) and model based variation (NormFinder) analysis of the top 100 genes with the lowest CV. The log2 transformed values of the average signal intensities among the 24 arrays were shown as Mean. Thirteen of the twenty genes were Ribosomal Protein Genes (Bold). Both ACTB and GAPDH were included for comparison but were not among the top 100 genes recommended by either statistical analysis.

Zhou et al. BMC Genomics 2010 11:75   doi:10.1186/1471-2164-11-75

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