Additional file 5.

Normalized target gene expression regulation in PC12 cells differentiated with GDNF, Forskolin and Y27632. Fold changes in transcript expressions of Egr-1 (i), Integrin alpha 1, ITGA1 (ii), and Crystallin alpha b, CRYAB (iii), in GDNF-GFRa1a-RET9 (A), GDNF-GFRa1a-RET51 (B), Forskolin (C), Y27632 (D) treated samples relative to that of control were normalized by geometric mean of top 2 reference genes in each subgroup; geometric mean of RPL19/RPL29; ACTB or GAPDH. Normalization by ACTB resulted in the over-estimation of target gene expression. Normalization by GAPDH led to either under- or over-estimation of target gene expression. Dotted line represents the 2-fold difference between treatment and control subjects, a cut off commonly used to distinguish significant changes from insignificant ones. Significant differences between fold changes normalized by various reference gene(s) were calculated using the paired Student's t test. A value of p < 0.05 was considered significant (**p < 0.01; *p < 0.05)

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Zhou et al. BMC Genomics 2010 11:75   doi:10.1186/1471-2164-11-75