Screenshot of final web output following a completed Southern blot probe design, search and analysis run. In this example a probe is designed against a 3 kb window on chromosome 2 of the NCBI37 mouse genomic assembly. 458 candidate probes were tested and many unique probes were found, 301/458 (shown in yellow). The remainder (157/458) passed the empirical cut-off criteria (shown in green) suggesting this region of the chromosome is likely composed of quite unique sequence, almost free from repetitive and low-complexity DNA elements.
Croning et al. BMC Genomics 2010 11:74 doi:10.1186/1471-2164-11-74