Additional file 1.

Figure S1. Realtime PCR validation of the miSeq libraries. Realtime PCR validation of the miSeq libraries prepared from heart tube and whole embryo RNA using the modified SREK protocol. Transcript specific forward primers for miR20b-5p, mir-206-3p and miR133 were based on full length miRbase [2] sequences and used in combination with a universal reverse primer that was designed to anneal to the 3' of the miSeq library as indicated in Figure 1A. miRNA expression was expressed relative to the total library content by using a forward primer at the 5' of the miSeq library.

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Buermans et al. BMC Genomics 2010 11:716   doi:10.1186/1471-2164-11-716