Resolution:
## Figure 3.
EM and HT miRNA expression. A: Venn diagram indicating the number of 5p (blue) and 3p (red) miRNA transcripts
expressed with at least 5 tpm in either HT or whole embryo libraries. B: Scatter plots
comparing the single (x-axis) and multiplex (y-axis) sequencing run for the sum of
isomirs per transcripts to calculate miRNA transcript expression levels in sqrt(tpm).
Heart tube and Embryo samples are indicated with triangles and diamonds, respectively.
Pearson correlation coefficients are indicated at the top of the plot for each summation
method. C: Heatmap clustering comparing all samples used in this study. All expressed
miRNA transcripts were used to generate this heatmap. Indicated at the right are the
tissues (EM & HT), total (tot) or small enriched (s) RNA, the single or multiplex
runs. The different summation methods to calculate miRNA transcript expression levels
for each sample, i.e., the sum of all isomirs, the sum of uniquely aligned isomirs
without mismatches and the most abundant isomir, are indicated with '_sum', '_U0'
and '_mab', respectively. Horizontal and vertical labels are identical. D: Scatter
plot indicating average miRNA expression in sqrt(tpm) for heart tube (x-axis) and
whole embryo (y-axis). Open and closed black circles represent non-significant and
significantly differentially expressed miRbase miRNA transcripts respectively. The
top left insert depicts an enlarged section of the 0-20 sqrt(tpm) area.
Buermans |