Table 1

Phenotypes of the parental, F1, and F2 mice (males).

B6 (n)

F1 (n)

TH (n)

F2 (n)

(lowest, highest)

16 F2 mice chosen for microarray

Lower (n = 8); Upper (n = 8)


Triglyceride (mg/dl)

8 wk

63 ± 12 (9)a

153 ± 12 (15)b

438 ± 41 (7)c

168 ± 3 (368) (60, 472)

130 ± 14; 207 ± 22 (P = 0.009)

12 wk

68 ± 8 (14)a

186 ± 11 (19)b

237 ± 53 (9)b

166 ± 3 (382) (44, 357)

135 ± 14; 230 ± 22 (P = 0.003)

16 wk

63 ± 3 (18)a

168 ± 8 (19)b

438 ± 39 (16)c

167 ± 4 (384) (20, 486)

120 ± 15; 191 ± 27 (P = 0.038)

20 wk

71 ± 7 (18)a

180 ± 20 (19)b

367 ± 32 (16)c

181 ± 4 (377) (56, 479)

126 ± 17; 336 ± 24 (P < 0.0001)

24 wk

60 ± 6 (18)a

221 ± 19 (19)b

368 ± 33 (16)c

181 ± 4 (375) (40, 436)

81 ± 7; 331 ± 17 (P < 0.0001)


Cholesterol (mmol/l)

8 wk

2.9 ± 0.2 (9)a

2.3 ± 0.1 (15)b

4.0 ± 0.1 (7)c

2.9 ± 0.1 (365) (0.7, 7.6)

2.2 ± 0.2; 2.9 ± 0.4 (P = 0.09)

12 wk

2.6 ± 0.2 (14)a

3.1 ± 0.2 (19)b

2.9 ± 0.1 (9)b

3.1 ± 0.1 (378) (0.4, 8.5)

2.5 ± 0.3; 3.1 ± 0.5 (P = 0.36)

16 wk

3.0 ± 0.3 (19)a

2.6 ± 0.1 (19)a

3.6 ± 0.3 (16)b

3.0 ± 0.1 (384) (1.0, 6.8)

2.5 ± 0.3; 3.6 ± 0.4 (P = 0.04)

20 wk

2.1 ± 0.1 (18)a

3.5 ± 0.3 (19)b

4.7 ± 0.3 (16)c

3.3 ± 0.1 (377) (1.1, 6.8)

2.7 ± 0.3; 3.6 ± 0.2 (P = 0.03)

24 wk

1.9 ± 0.1 (18)a

3.6 ± 0.1 (19)b

4.0 ± 0.4 (16)b

3.3 ± 0.1 (375) (1.3, 8.1)

2.8 ± 0.2; 3.1 ± 0.5 (P = 0.58)


Glucose (mg/dl)

8 wk

175 ± 10 (9)a

175 ± 11 (15)a

292 ± 48 (7)b

176 ± 2 (368) (98, 353)

163 ± 11; 183 ± 9 (P = 0.20)

12 wk

132 ± 6 (14)a

147 ± 9 (19)a

252 ± 40 (9)b

166 ± 2 (382) (45, 473)

145 ± 8; 168 ± 11 (P = 0.10)

16 wk

181 ± 14 (19)a

166 ± 4 (19)a

460 ± 38 (16)b

169 ± 3 (384) (62, 575)

144 ± 15; 160 ± 11 (P = 0.40)

20 wk

177 ± 10 (18)a

156 ± 5 (19)a

364 ± 36 (16)b

165 ± 3 (378) (64, 492)

144 ± 17; 163 ± 13 (P = 0.40)

24 wk

175 ± 10 (18)a

162 ± 8 (19)a

387 ± 33 (16)b

162 ± 3 (375) (40, 599)

131 ± 9; 128 ± 13 (P = 0.83)


Insulin (ng/ml)

8 wk

0.07 ± 0.02 (9)a

0.96 ± 0.17 (15)b

1.81 ± 0.41 (7)c

1.31 ± 0.06 (384) (0.01, 9.71)

1.45 ± 0.31; 1.36 ± 0.28 (P = 0.83)

12 wk

0.22 ± 0.07 (13)a

1.12 ± 0.15 (19)b

0.98 ± 0.33 (9)b

1.55 ± 0.08 (376) (0.06, 12.36)

0.80 ± 0.14; 1.28 ± 0.17 (P = 0.05)

16 wk

0.35 ± 0.09 (18)a

1.24 ± 0.13 (19)b

0.98 ± 0.26 (15)b

2.17 ± 0.11 (381) (0.07, 15.03)

1.19 ± 0.23; 1.94 ± 0.43 (P = 0.20)

20 wk

0.42 ± 0.11 (18)a

2.14 ± 0.26 (19)b

1.56 ± 0.41 (16)b

2.39 ± 0.15 (376) (0.02, 23.18)

1.12 ± 0.36; 2.35 ± 0.47 (P = 0.06)

24 wk

0.41 ± 0.10 (10)a

2.78 ± 0.55 (16)b

2.31 ± 0.56 (13)b

2.70 ± 0.16 (371) (0.06, 23.97)

2.59 ± 1.10; 5.13 ± 2.10 (P = 0.30)


Body weight (g)

8 wk

22 ± 0.4 (14)a

30 ± 0.5 (15)b

31 ± 0.5 (7)b

29 ± 0.2 (385) (20, 40)

30 ± 1.1; 29 ± 0.9 (P = 0.33)

12 wk

24 ± 0.5 (18)a

33 ± 0.6 (19)b

32 ± 0.6 (16)b

33 ± 0.2 (385) (23, 45)

33 ± 1.2; 33 ± 1.2 (P = 0.65)

16 wk

25 ± 0.5 (18)a

36 ± 0.7 (19)b

34 ± 0.8 (16)b

35 ± 0.2 (383) (24, 49)

35 ± 1.5; 36 ± 1.3 (P = 0.66)

20 wk

27 ± 0.5 (18)a

38 ± 0.7 (19)b

36 ± 1.0 (16)c

37 ± 0.3 (378) (25, 52)

36 ± 2.0; 38 ± 1.1 (P = 0.39)

24 wk

28 ± 0.4 (18)a

41 ± 0.6 (19)b

36 ± 1.4 (16)c

39 ± 0.3 (373) (25, 59)

38 ± 2.2; 41 ± 1.3 (P = 0.26)


Fat pad & Carcass weights (g)

IG

0.34 ± 0.02 (18)a

1.37 ± 0.07 (19)b

0.69 ± 0.15 (16)c

0.99 ± 0.03 (372) (0.07, 2.66)

0.64 ± 0.14; 1.14 ± 0.10 (P = 0.01)

ED

0.43 ± 0.03 (18)a

1.86 ± 0.07 (19)b

0.98 ± 0.23 (16)c

1.41 ± 0.03 (372) (0.19, 3.21)

1.11 ± 0.25; 1.69 ± 0.19 (P = 0.08)

MS

0.11 ± 0.01 (18)a

0.60 ± 0.03 (19)b

0.24 ± 0.06 (16)c

0.40 ± 0.01 (372) (0.07, 1.30)

0.35 ± 0.12; 0.46 ± 0.04 (P = 0.40)

RP

0.12 ± 0.01 (18)a

0.64 ± 0.02 (19)b

0.26 ± 0.06 (16)c

0.43 ± 0.01 (371) (0.05, 1.47)

0.31 ± 0.07; 0.63 ± 0.13 (P = 0.05)

SC

0.14 ± 0.01 (18)a

0.72 ± 0.04 (19)b

0.32 ± 0.09 (16)c

0.50 ± 0.02 (372) (0.05, 3.26)

0.32 ± 0.08; 0.59 ± 0.06 (P = 0.01)

Sum

1.14 ± 0.06 (18)a

5.17 ± 0.20 (19)b

2.34 ± 0.55 (16)c

3.72 ± 0.09 (371) (0.52, 8.59)

2.69 ± 0.61; 4.51 ± 0.31 (P = 0.02)

Carcass

26 ± 0.4 (18)a

34 ± 0.5 (19)b

31 ± 1.0 (16)c

33 ± 0.2 (371) (23, 47)

34 ± 1.8; 34 ± 1.0 (P = 0.91)


Data are presented as mean ± sem. Means labeled with different letters are significantly different from one another comparing B6, F1 and TH (P < 0.05). wk, week; IG, inguinal; ED, epididymal; MS, mesenteric; RP, retroperitoneal including perirenal; SC, subscapular; Sum, sum of the 5 fat pads above; B6, C57BL/6J; TH, TALLYHO/JngJ.

Stewart et al. BMC Genomics 2010 11:713   doi:10.1186/1471-2164-11-713

Open Data