Anaysis of selected novel transcripts by reverse transcritption (RT)-PCR and Southern blot analysis. A. Expression of selected ESTs in O. longistaminata roots. M: DNA marker; 1: Xa21_5; 2: Xa21_11*; 3: Xa21_18; 4: Xa21_140*; 5: Xa21_202*; 6: Xa21_289; 7: Xa21_1050*; 8: Xa21_3892; 9: Xa21_20888; 10: Xa21_26755*. * indicated that those ESTs could also be amplified from root cDNAs of cultured wild rice seeds. B. Southern blotting analysis of novel ESTs. M: DNA marker; 1: O. longistaminata genomic DNA; 2: IR36 genomic DNA; 3: Nipponbare genomic DNA.
Yang et al. BMC Genomics 2010 11:705 doi:10.1186/1471-2164-11-705