Additional file 2.

Transcripts with different representation between the larval library and the combined adult libraries. To calculate the fold difference in the representation of unigenes in our libraries, we used the number of reads from each library normalized by the library size (in number of reads) as a measure of the transcript abundance. Of the 645 unigenes differently represented, 356 had no read in one of the libraries and the fold change was calculated assuming a value of 1. Positive and negative values represent a highest number of reads in the adult and larva libraries, respectively. Only unigenes represented by at least 20 reads were included.

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Carvalho et al. BMC Genomics 2010 11:695   doi:10.1186/1471-2164-11-695