Additional file 4.

Amino acid sequence alignment of Imaginal disc Growth Factors (IDGFs)-like proteins from different insect species. The sequence of the Ci-48b from C. inanitus was aligned with sequences from the following species: N. vitripennis [GenBank:XP_001599305.1], A. mellifera [GenBank:XP_396769.2] and Manduca sexta [GenBank:ACW82749.1]. The conserved region II is boxed. Triangle indicates a glutamine residue replacing, in these proteins, a glutamic acid residue of functional importance. Location of the glycosyl hydrolase family 18 domain is indicated by a blue line. Red and green arrows indicate the beginning of the predicted signal peptide and mature protein sequences of Ci-48b, respectively. Sequence printed in bold was also obtained by N-terminal sequencing of the Ci-48b protein.

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Vincent et al. BMC Genomics 2010 11:693   doi:10.1186/1471-2164-11-693