Additional file 3.

Amino acid sequence alignment of representative chitinases from different insect species. The sequence of the Ci-45 venom chitinase from C. inanitus was aligned with sequences of chitinases from the following species: the parasitic wasps C. sp. near curvimaculatus [GenBank:AAA61639.1], T. nigriceps [GenBank:AAX69085.1] and N. vitripennis [GenBank:NP_001128139.2] and the beetle Monochamus alternatus [GenBank:BAF49605.1]. The four conserved regions are boxed. Black triangles indicate catalytic residues. Locations of the glycosyl hydrolase family 18 and chitin-binding Peritrophin-A (CBM_14) domains are indicated by blue and green lines, respectively. Red and green arrows indicate the beginning of the predicted signal peptide and mature protein sequences of Ci-45, respectively. Sequence printed in bold was also obtained by N-terminal sequencing of the Ci-45 protein.

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Vincent et al. BMC Genomics 2010 11:693   doi:10.1186/1471-2164-11-693