Additional file 3.
PCR anaslysis of three TR4 binding sites. ChIP assays were performed in K562 and HepG2 cells using TR4 antibody. PCR was performed using primers to TR4 binding sites identified by ChIP-seq (see Additional file 2 for oligo sequences). The enrichment of TR4 is shown in comparison to 0.1% Input chromatin. IgG ChIPs were used as negative controls. PCR analysis confirmed presence of TR4 at TNFIAP1, ECSIT and SCAP, but showed no significant enrichment when using negative control primers to ZNF333.
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O'Geen et al. BMC Genomics 2010 11:689 doi:10.1186/1471-2164-11-689