Quantification of FOX2 transcript variant expression. (a) Four of the annotated transcript variants of FOX2 (for complete set, see Figure 6a) and location of qRT-PCR assays that measure gene level expression and transcript variant expression, respectively. (b) Location of primers for transcript variant specific qRT-PCR assays in the C-terminal region of FOX2 (green: constitutive exons; orange: cassette exon; grey: cassette exons not expressed; green arrows: primers; dotted arrow: junction primer). (c) Quantification of gene expression (G) and transcript variant expression (CE: cassette exon; ES: exon skipping) in adenocarcinoma and squamous cell carcinoma of NSCLC (red bars), respectively, compared to NAT (blue bars). Bars indicate median ΔCt values, dots represent values for individual samples (AdCa: n = 6; SCC: n = 4), error bars indicate one standard deviation. (d) Fold-change (FC) of over-expression in adenocarcinoma of NSCLC versus NAT and splicing index (SI). Median values based on six sample pairs are shown (values for each patient shown as dots), significance was determined using a paired t-test. (e) Fold-change of over-expression in squamous cell carcinoma of NSCLC versus NAT and splicing index. Median values based on four sample pairs are shown.
Langer et al. BMC Genomics 2010 11:676 doi:10.1186/1471-2164-11-676