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Open Access Highly Accessed Research article

Genome wide transcriptome profiling of a murine acute melioidosis model reveals new insights into how Burkholderia pseudomallei overcomes host innate immunity

Chui-Yoke Chin1, Denise M Monack2 and Sheila Nathan13*

Author Affiliations

1 School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi Selangor D. E. Malaysia

2 Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305-5120, USA

3 Malaysia Genome Institute, UKM-MTDC Technology Centre, 43600 UKM Bangi, Selangor D. E. Malaysia

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BMC Genomics 2010, 11:672  doi:10.1186/1471-2164-11-672

Published: 27 November 2010

Additional files

Additional file 1:

Figure S1 - Mortality of mice (n = 3-5 mice/group) infected intravenously with B. pseudomallei strain (A) D286, (B) H10 and (C) R15. Mice were infected intravenously with doses from 102 to 106 CFU. Animals were observed daily up to ten-days, and the percentage survival plotted against time.

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Additional file 2:

Table S1 - Kinetic profiles of host gene expression modulated by B. pseudomallei infection in both liver and spleen.

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Additional file 3:

Figure S2 - Transcriptional changes of genes involved in the complement system. Shown is the expression profile for genes modulated at 42 hpi in liver and spleen. Induced genes are highlighted in red while the repressed genes are highlighted in green.

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Additional file 4:

Figure S3 - Transcriptional changes of genes involved in the glycolysis and TCA pathways. Shown are the expression profiles for liver genes (24 hpi) encoding enzymes involved in glycolysis (left) and TCA cycle (right). Induced genes are highlighted in red while the repressed genes are highlighted in green.

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Additional file 5:

Table S2 - Primers used in qRT-PCR.

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