Additional file 5.
Verification of two-hybrid interacting pairs by co-immunoprecipitation. Additional file 5 contains four tables (S10 to S13) listing interacting proteins pairs tested and results. Supplemental Table S10 lists tested pairs involving PDZ domains and their respective two-hybrid identified interacting proteins possessing a free C-terminus. Supplemental Table S11 lists tested pairs involving PDZ domains and their respective two-hybrid identified interacting proteins using a B2 tailed construct. Supplemental Table S12 lists the 59 protein fragments that did not possess a C-terminal binding motif tested by co-immunoprecipitation in a C-terminally truncated form against their respective two hybrid interacting PDZs. Sequences where there was creation of a new C-terminal consensus sites after truncation are shown. Supplemental Table S13 lists the primers pairs used to amplify and clone truncated interacting protein fragments used in the co-immunoprecipitation experiment.
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Lenfant et al. BMC Genomics 2010 11:671 doi:10.1186/1471-2164-11-671