Additional file 3.
PDZome network. Additional file 3 contains four tables (S3 to S6) listing the PDZome network interacting pairs and giving statistical analysis of the interactions. Supplemental Table S3: Two hybrid screen results. Gene names and ID are given according to Wormbase WS150. Number of hits refers to the number of independent colonies identified and phenotypically tested for each interacting partner. Number of splice-forms identified or predicted in Wormbase WS150 and last 6 amino acids of each splice-form are given in cases were several splice-forms are identified or predicted. When sequencing from the N-terminus did not span the entire fragment, and thus the C-terminus was not experimentally confirmed, if any of the predicted splice-form had a C-terminal consensus motif, to be conservative, a consensus class was attributed. Consensus class type: [ST]X[YFWCMVILA] = 1; [YFWCMVILA]X[YFWCMVILA] = 2; [DE]X[YFWCMVILA] = 3 (X: any amino acid). Supplemental Table S4: Promiscuity and specificity of PDZ interactome network. Number of independent interacting proteins per PDZ domain, and number of PDZ domains interacting with each protein are given for AD-wrmcDNA and AD-ORFeome libraries two-hybrid screens. Supplemental Table S5: Promiscuity and specificity of PDZ interactome network: mean and median for number of interacting proteins per PDZ or vice versa. Supplemental Table S6: Number of interacting proteins per consensus class in network. Single hits: interacting proteins for which only one clone was identified in two-hybrid screens. Multiple hits: interacting proteins for which more than one clone was identified in two-hybrid screens.
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Lenfant et al. BMC Genomics 2010 11:671 doi:10.1186/1471-2164-11-671