Role of nucleotide sequence around the ERSE motif in mouse CRELD2 and ALG12 promoters in regulating the transcriptional activity of both genes. Neuro2a cells were transfected with the indicated CRELD2 or ALG12 reporter constructs. Twenty-four hours after transfection, the cells were incubated with or without Tg (0.1 μM) for 10 h (A and C). The expression vector for ATF6 or an empty vector (mock) was co-transfected with each reporter construct into Neuro2a cells and cultured for 36 h (B and D). After incubation, the cells were lysed and luciferase activity was measured as described in methods. Control and Tg-treated or ATF6-stimulated activities are shown as open and closed columns, respectively. Values represent means ± SD from 3 - 4 independent cultures and are expressed relative to the basal activity of the pGL3-Promoter (A and B) and pGL3-Basic (C and D).
Oh-hashi et al. BMC Genomics 2010 11:664 doi:10.1186/1471-2164-11-664