Table 2

Accuracy and precision of platforms across linear range

Platform


Standards

Microarray

RT-qPCR


Slope

R2

Slope

R2


13

1.0566

0.9991

1.0004

0.9988


42

1.0047

0.9675

1.0205

0.9988


81

1.0389

0.9671

0.9946

0.9994


84

0.9772

0.9639

0.9693

0.9989


95

0.957

0.9712

1.0022

0.9993


99

1.0095

0.9626

1.0052

0.9994


113

1.0248

0.9993

1.0200

0.9970


171

1.0696

0.9993

0.9714

0.9988


The accuracy and precision of the platforms across the linear range (Microarray: 10-105 copies/ng; RT-qPCR: 10-106 copies/ng) were assessed by the slope (ideal slope = 1.000) and Pearson correlation coefficient (R2) values respectively, based on linear regression of transcript copies/ng vs. normalised signal intensity (microarray) or ΔCt values (RT-qPCR). RT-qPCR values were adjusted for the PCR efficiency of the assay.

Devonshire et al. BMC Genomics 2010 11:662   doi:10.1186/1471-2164-11-662

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