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Open Access Highly Accessed Research article

Evaluation of external RNA controls for the standardisation of gene expression biomarker measurements

Alison S Devonshire, Ramnath Elaswarapu* and Carole A Foy

Author Affiliations

LGC Limited, Queens Road, Teddington, Middlesex, TW11 0LY, UK

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BMC Genomics 2010, 11:662  doi:10.1186/1471-2164-11-662

Published: 24 November 2010

Additional files

Additional file 1:

Information about RNA sequences and RT-qPCR assays. This Microsoft Word file gives details of ERCC RNA sequences, microarray probes and Taqman® assays.

Format: DOC Size: 52KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 2:

Linear range of detection on microarray and RT-qPCR platforms. This Microsoft Powerpoint file presents data related to correlation of transcript copy number with normalized microarray signal output and RT-qPCR ΔCt values for all standards investigated.

Format: PPT Size: 1MB Download file

This file can be viewed with: Microsoft PowerPoint Viewer

Open Data

Additional file 3:

Results of correlation analysis of whole array data. Microsoft Word file displays data from pairwise correlation of microarray data (all entities) from three replicate microarrays.

Format: DOC Size: 57KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data