Validation of microarray data by RT-PCR. Relative gene expression of arbitrarily selected genes in HaCaT cells after 3 d of exposure to 30 μg/ml WC and 33 μg/ml WC-Co nanoparticles and 3 μg/ml CoCl2 was analysed by semiquantiative RT-PCR. Selected genes represent genes with significant changes (2 to 23fold) of expression levels in microarrays. Gene expression values were converted to percent of the mean of controls and are presented as mean + standard deviation (SD). Statistical differences were analysed with one-way ANOVA followed by Dunnett's post test (treatment vs. control). Values of p < 0.05 were considered statistically significant; *p < 0.05, **p < 0.01.
Busch et al. BMC Genomics 2010 11:65 doi:10.1186/1471-2164-11-65