Open Access Research article

Differences in the transcriptome signatures of two genetically related Entamoeba histolytica cell lines derived from the same isolate with different pathogenic properties

Laura Biller1, Paul H Davis2, Manuela Tillack1, Jenny Matthiesen1, Hannelore Lotter1, Samuel L Stanley3, Egbert Tannich1 and Iris Bruchhaus1*

Author Affiliations

1 Bernhard Nocht Institute for Tropical Medicine, Bernhard-Nocht-Str. 74, 20359 Hamburg, Germany

2 Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania, USA

3 Office of the President, Stony Brook University, Stony Brook, New York, USA

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BMC Genomics 2010, 11:63  doi:10.1186/1471-2164-11-63

Published: 26 January 2010

Additional files

Additional file 1:

List of genes differentially transcribed in E. histolytica HM-1:IMSS cell lines A and B identified by microarray analyses. Using an microarray and analyzing two biological replicates, 87 gene transcripts were detected that show a two-fold or greater difference in expression between cell line A and cell line B. Out of these, 47 genes were significantly upregulated in the non-pathogenic cell line A and 40 genes were transcribed at significantly higher levels in the pathogenic cell line B.

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Additional file 2:

List of oligonucleotides used for real-time PCR. Quantitative real-time PCR was used to confirm the differential transcription of 27 selected genes that showed at least a three-fold higher level of transcription in one or other cell line.

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