Table 4

Bacterial strains and plasmids

Strain or plasmid

Description

Source or reference


Strain

F. tularensis

LVS

subsp. holarctica, live vaccine strain

A. Sjöstedt

LVSΔftrA

LVS with deletion of ftrA

This study

LVSΔftrB

LVS with deletion of ftrB

This study

E. coli

DH5α

Strain collection

Plasmid

pCR2.1-TOPO

PCR cloning vector, KmR, AmpR

Invitrogen

pMP812

sacB suicide vector, KmR

[40]

pMP-ΔftrA

pMP812 containing ~2 kb fragment for deletion of ftrA

This study

pMP-ΔftrB

pMP812 containing ~2 kb fragment for deletion of ftrB

This study


Postic et al. BMC Genomics 2010 11:625   doi:10.1186/1471-2164-11-625

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