Table 1

qRT-PCR validation of microarray data

Gene Symbol

Microarray fold change

qRT-PCR fold change

Correlation*

1 hr

3 hr

6 hr

24 hrs

1 hr

3 hr

6 hr

24 hrs


IL1B

4.9

14.6

5.7

1.7

41.0

127.4

52.6

5.2

0.99

IL6

103.3

771.7

193.0

15.2

6.4

11.9

6.1

1.4

0.92

IL8

44.0

311.9

231.6

454.6

21.7

244.5

293.8

477.4

0.96

TNF

3.4

1.3

1.5

1.3

5.2

2

2.5

0.8

0.95

IL10

2.2

2.1

2.8

2.3

1.8

1.7

1.9

0.7

0.4

IL18

1.1

1.1

1.4

1.3

1.4

1.3

1.6

0.7

0.68

TGFBI

1.5

1.3

1.5

3.2

1.6

1.7

1.7

3.2

0.99

TLR4

1.3

2.3

2.0

1.2

0.8

1.5

1.2

0.5

0.96

TLR2

2.0

4.6

3.4

2.8

1.5

2.7

2.3

1.0

0.83

IL4

4.2

19.5

28.8

2.2

1.9

8.7

9.2

0.8

0.97

CSF2

6.7

4.9

4.4

1.6

9.2

9.1

9.8

1.4

0.86

Mean correlation

0.87


Key - Comparisons between baseline samples (time = 0) and individual time points. *Correlations performed using Pearson's correlation co-efficient for highly expressed genes, i.e. IL1B, IL6, IL8, TNF, TLR4, TLR2, IL4 and CSF2 and Spearman's rank correlation co-efficient for genes with lower expression levels, i.e. IL10, IL18 and TGFBI

Burgess et al. BMC Genomics 2010 11:624   doi:10.1186/1471-2164-11-624

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