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Open Access Highly Accessed Research article

Digital gene expression tag profiling of bat digits provides robust candidates contributing to wing formation

Zhe Wang12, Dong Dong13, Binghua Ru1, Rebecca L Young2, Naijian Han4, Tingting Guo1 and Shuyi Zhang1*

Author Affiliations

1 Institute of Molecular Ecology and Evolution, iAIR, East China Normal University, Shanghai 200062, PR China

2 Yale Systems Biology Institute, Department of Ecology and Evolutionary Biology, Yale University, West Haven, CT 06516, USA

3 Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, ON, M5S 3E1, Canada

4 Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, PR China

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BMC Genomics 2010, 11:619  doi:10.1186/1471-2164-11-619

Published: 6 November 2010

Additional files

Additional file 1:

Table S1.xls. Tag frequency. The number of distinct and total tags counted at different frequencies in each library. FRQ indicates the frequency of distinct tags in the library.

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Additional file 2:

Table S2.xls. Expression and analysis of human gene homologs in bat digits The expression of human gene homologs mapped by tags in each library and the results of DEGseq analysis for the study of gene differential expression [56]. Symbol indicates NCBI official gene symbol; GeneID indicates NCBI Entrez Gene ID. The raw count of each mapped gene is presented under its library name (Libraries Hand DI, Hand DII-V or Foot). All other parameters (log2(fold_change), log2(fold_change) nomalized, z-score, p-value, q-value and signature) were calculated using the DEGseq [56].

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Additional file 3:

Table S3.xls. Expression and analysis of mouse gene homologs in bat digits The expression of mouse gene homologs mapped by tags in each library and the results of DEGseq analysis for the study of gene differential expression [56]. Symbol indicates NCBI official gene symbol; GeneID indicates NCBI Entrez Gene ID. The raw count of each mapped gene is presented under its library name (Libraries Hand DI, Hand DII-V or Foot). All other parameters (log2(fold_change), log2(fold_change) nomalized, z-score, p-value, q-value and signature) were calculated using the DEGseq [56].

Format: XLS Size: 3.4MB Download file

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Additional file 4:

Table S4.xls. Sequences of primers used in PCR and RT-qPCR Nucleotide sequences of the primers used in PCR and RT-qPCR of candidate genes underlying digit distinct morphologies (Figure 5). a indicates previous published primers [57]. The lengths of introns are inferred from the homologs of human genes.

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