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Open Access Highly Accessed Research article

Gene expression in Pseudomonas aeruginosa swarming motility

Julien Tremblay and Eric Déziel*

Author Affiliations

INRS-Institut Armand-Frappier, Laval (Québec), H7V 1B7, Canada

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BMC Genomics 2010, 11:587  doi:10.1186/1471-2164-11-587

Published: 20 October 2010

Additional files

Additional file 1:

Swarming motility video clip. Movie clip of Pseudomonas aeruginosa PA14 swarming motility on M9DCAA medium solidified with 0.5% agar. The plate was incubated at 30°C. Video clip was constructed by assembling images taken every 3 minutes over a period of 21 hrs. and 45 min. with a Canon 10D camera equipped with a Canon Ultrasonic 28-80 mm lens.

Format: AVI Size: 5.7MB Download file

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Additional file 2:

Differentially expressed genes of tendril tip, swarm center and non-swarming bacterial populations. The complete list of genes differentially regulated in log2 fold-change with a p-value of 0.05 or lower.

Format: XLS Size: 220KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 3:

List of primers used in the qRT-PCR experiments. Complete lists of primers used in the qRT-PCR experiments, including primer sequences are shown.

Format: XLS Size: 21KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 4:

Venn diagram representing differentially expressed genes for each conditions and possible combinations. Genes in each categories (A to G) are listed in additional file #2 (excel file) under the "summary" tab. (↑) up-regulated genes; (↓) down-regulated genes. Group A: 58 genes up- and 134 down-regulated in tendril tip vs. non-swarming. Group B: 16 genes up- and 3 down-regulated in swarm center vs. non-swarming. Group C: 4 genes up- and 22 down-regulated in tendril tip vs. swarm center. Group D: 2 genes up- and 27 down-regulated in tendril tip vs. non-swarming and swarm center vs. non-swarming. Group E: 15 genes up- and 59 down-regulated in tendril tip vs. non-swarming and tendril tip vs. swarm center. Group F: *25 genes are down-regulated in tendril tip vs. swarm center while being up-regulated in swarm center vs. non-swarming control. Group G: 13 genes are down-regulated in tendril tip vs. non-swarming, tendril tip vs. swarm center and swarm center vs. non-swarming.

Format: EPS Size: 781KB Download file

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Additional file 5:

Flagella observations. Typical images of bacterial cells isolated from tendril tip, swarm center and non-swarming conditions. The flagella staining procedure was performed as described by Merritt and coworkers [96].

Format: EPS Size: 1.2MB Download file

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Additional file 6:

Swarm center population contains more dead cells than non-swarming bacteria. Swarming and non-swarming colonies were grown as described in material and methods. For each conditions (swarm center and non-swarming), ten circular agar plugs of 0.75 cm diameter containing swarm center and non-swarming bacteria were extracted from plates and vigorously resuspended in 2 mL of sterile PBS buffer and serially diluted. For CFU count, 100 μL of each serial dilution was plated on TSB agar plates and incubated O/N at 37°C. To determine the dry weight, the remaining bacterial suspensions were placed in pre-weighed aluminum cups and incubated at 65°C for 4 hrs to allow water evaporation. Cups were weighed again to determine total dry weight. All experiments were performed in triplicates.

Format: EPS Size: 529KB Download file

Open Data