Open Access Research article

Gene expression profiling to identify eggshell proteins involved in physical defense of the chicken egg

Vincent Jonchère1, Sophie Réhault-Godbert1, Christelle Hennequet-Antier1, Cédric Cabau1, Vonick Sibut13, Larry A Cogburn2, Yves Nys1 and Joel Gautron1*

Author Affiliations

1 INRA, UR83 Recherches Avicoles, F-37380 Nouzilly, France

2 Department of Animal and Food Sciences, University of Delaware, Newark, DE 19717 USA

3 Institut Technique Avicole, F-37380 Nouzilly, France

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BMC Genomics 2010, 11:57  doi:10.1186/1471-2164-11-57

Published: 21 January 2010

Additional files

Additional file 1:

Differentially expressed transcripts in uterus vs. magnum or white isthmus. Excel file describing the differentially expressed transcripts in the comparison uterus vs. magnum (UT/Ma), uterus vs. white isthmus (Ut/WI) and 605 common genes between both contrast.

Format: XLS Size: 1.8MB Download file

This file can be viewed with: Microsoft Excel Viewer

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Additional file 2:

Comparison of gene expression using microarray and qRT-PCR analyses. Word file giving numerical and statistical data of the gene which were validated using qRT-PCR

Format: DOC Size: 50KB Download file

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Additional file 3:

Correspondence between differentially expressed uterine genes and proteomic analysis of eggshell proteins. The Excel file lists the uterine specific cDNA sequences and their correspondences with the eggshell proteins already reported, determined using BlastX. Exponentially modified protein abundance index (EmPAI) is an estimation of protein abundance based on the number of sequenced peptides in the proteomic study.

Format: XLS Size: 45KB Download file

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Additional file 4:

List of primers used for RT-PCR. Word file where are mentioned the primer sequences used in this study

Format: DOC Size: 42KB Download file

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